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Published: September 11, 2022 (2 weeks ago)
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#re-loader #reloader activator 3.3download.os #reloader #reloader activator v1.6 final office and windows 10..]] Similarity factors were calculated as the mean distance between sequences in a tree (full tree length / 2) for both ML and neighbor joining analyses.

Calculation of the evolutionary distances was achieved using the NJ method with 100 bootstrap replicates in MEGA4 ([[@B25]]).

Estimation of divergence times
——————————

The most likely number of transfer events between potential donor and recipient genomes was estimated using the total branch lengths as described elsewhere [[@B12]] and allowed for heterogeneous patterns of transfer events along the evolutionary history of the recipient species.

Four independent simulations were conducted for each host-parasite pair using the Tree Simulation program of the NSR data analysis package. Branch lengths were determined from the ML tree constructed from all loci. For each pair, 15,000 separate replicates were calculated using either the ML or NJ trees derived from each of the four loci. Branch lengths were sampled from the values observed in the trees after discarding the first 5,000,000 iterations of the run.

Authors’ contributions
=======================

BD carried out the phylogenetic and evolutionary analyses. All authors conceived the study, participated in the design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.

Acknowledgements
================

The authors wish to thank Valentina D’Souza for field sampling, Ricardo D’Amelio, Mariangela Righini and Gabriella Marinelli for help with sequencing, Francisca Tovar for technical help. Anne Kneib is thanked for helpful discussions. This work was supported by the German Academic Exchange Service and by the European Union within the EUVAC (EVAg) grant ANTIVIRAEVAC-2 (Antiviral Control in the European Animal Health Community).
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